DDX58 Rabbit Polyclonal Antibody

CAT#: TA375406

RIG-I / DDX58 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using RIG-I / DDX58 antibody (TA375406) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of H9C2 cells using RIG-I / DDX58 antibody (TA375406) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using RIG-I / DDX58 antibody (TA375406) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U20S cells using RIG-I / DDX58 antibody (TA375406) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 107kDa
• Gene Name: DEXD/H-box helicase 58
• Database Link:
  Entrez Gene 23586 Human
  UniProt ID O95786
• Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
• Synonyms: DKFZp434J1111; DKFZp686N19181; FLJ13599; RIG-1; RIG-I