C20orf31 (EDEM2) Rabbit Polyclonal Antibody

CAT#: TA375710

EDEM2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using EDEM2 Rabbit pAb (TA375710) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

• 

  Immunofluorescence analysis of L929 cells using EDEM2 Rabbit pAb (TA375710) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 65kDa
• Gene Name: ER degradation enhancing alpha-mannosidase like protein 2
• Database Link:
  Entrez Gene 55741 Human
  UniProt ID Q9BV94
• Background: In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).
• Synonyms: bA4204.1; C20orf31; C20orf49; FLJ10783