PKR (EIF2AK2) Rabbit Polyclonal Antibody

CAT#: TA375789

Phospho-PKR/EIF2AK2-T446 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Phospho-PKR/EIF2AK2-T446 antibody (TA375789) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho T446
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding T446 of human PKR/EIF2AK2
• Formulation: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 62kDa
• Gene Name: eukaryotic translation initiation factor 2 alpha kinase 2
• Database Link:
  Entrez Gene 5610 Human
  UniProt ID P19525
• Background: The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: EIF2AK1; MGC126524; OTTHUMP00000201320; PKR; PRKR