EIF4G1 Rabbit Polyclonal Antibody

CAT#: TA375837

Phospho-EIF4G1-S1108 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EIF4G1-S1108 antibody (TA375837) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 60s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S1108
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S1108 of human EIF4G1 (NP_001181875.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 154kDa/158kDa/166kDa/171kDa/175kDa/176kDa
• Gene Name: eukaryotic translation initiation factor 4 gamma 1
• Database Link:
  Entrez Gene 1981 Human
  UniProt ID Q04637
• Background: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
• Synonyms: DKFZp686A1451; eIF-4G1; EIF4-gamma; EIF4F; EIF4G; EIF4GI; p220