NSE (ENO2) Rabbit Polyclonal Antibody

CAT#: TA375897

ENO2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ENO2 antibody (TA375897) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of L929 cells using ENO2 antibody (TA375897) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using ENO2 antibody (TA375897) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 47kDa
• Gene Name: enolase 2
• Database Link:
  Entrez Gene 2026 Human
  UniProt ID P09104
• Background: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
• Synonyms: NSE