Fibrillarin (FBL) Rabbit Polyclonal Antibody

CAT#: TA376140

Fibrillarin/U3 RNP Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Fibrillarin/U3 RNP antibody (TA376140) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.

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  Immunofluorescence analysis of HeLa cells using Fibrillarin/U3 RNP antibody (TA376140) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of C6 cells using Fibrillarin/U3 RNP antibody (TA376140) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using Fibrillarin/U3 RNP antibody (TA376140) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug Fibrillarin/U3 RNP antibody (TA376140). Western blot was performed from the immunoprecipitate using Fibrillarin/U3 RNP antibody (TA376140) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ELISA, ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat, Monkey
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 34kDa
• Gene Name: fibrillarin
• Database Link:
  Entrez Gene 2091 Human
  UniProt ID P22087
• Background: This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
• Synonyms: FIB; FIB1; fibrillarin; FLRN; RNU3IP1