Folate Binding Protein (FOLR1) Rabbit Polyclonal Antibody

CAT#: TA376314

Folate Binding Protein(FBP) / FOLR1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Rat brain, using Folate Binding Protein(FBP) / FOLR1 antibody (TA376314) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunohistochemistry of paraffin-embedded rat testis using Folate Binding Protein(FBP) / FOLR1 Rabbit pAb (TA376314) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human colon using Folate Binding Protein(FBP) / FOLR1 Rabbit pAb (TA376314) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of NIH-3T3 cells using Folate Binding Protein(FBP) / FOLR1 Rabbit pAb (TA376314) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using Folate Binding Protein(FBP) / FOLR1 Rabbit pAb (TA376314) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-93 of human Folate Binding Protein(FBP) / FOLR1 (NP_000793.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 29kDa
• Gene Name: folate receptor 1 (adult)
• Database Link:
  Entrez Gene 2348 Human
  UniProt ID P15328
• Background: The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
• Synonyms: FBP; FOLR; FR-alpha