FOXO1 Rabbit Polyclonal Antibody

CAT#: TA376337

FOXO1/FOXO3A/FOXO4 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using FOXO1/FOXO3A/FOXO4 antibody (TA376337) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunohistochemistry of paraffin-embedded rat brain using FOXO1/FOXO3A/FOXO4 Rabbit pAb (TA376337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human colon carcinoma using FOXO1/FOXO3A/FOXO4 Rabbit pAb (TA376337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse spinal cord using FOXO1/FOXO3A/FOXO4 Rabbit pAb (TA376337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human FOXO1/FOXO3A/FOXO4.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: forkhead box O1
• Database Link:
  Entrez Gene 2308 Human
  UniProt ID Q12778
• Background: The Forkhead family of transcription factors mediates many aspects of physiology, including stress response, metabolism, commitment to apoptosis, and development. The Forkhead box subfamily O (FoxO) proteins have garnered particular interest due to their involvement in the modulation of cancer, metabolism and cardiovascular biology.In mammals, four subfamily members have been identified: FOXO1 (previously also known as FKHR), FOXO3 (also known as FKHRL1), FOXO4 (also known as AFX),and FOXO6.
• Synonyms: FKH1; FKHR; FOXO1A