FOXO1 Rabbit Polyclonal Antibody

CAT#: TA376338

Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (TA376338) at 1:500 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (TA376338) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (TA376338) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (TA376338) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho T24,Phospho T32,Phospho T28
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho synthetic peptide corresponding to residues surrounding T24/T32/T28 of human FOXO1/FOXO3A/FOXO4.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: forkhead box O1
• Database Link:
  Entrez Gene 2308 Human
  UniProt ID Q12778
• Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
• Synonyms: FKH1; FKHR; FOXO1A