FUS Rabbit Polyclonal Antibody

CAT#: TA376385

FUS Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody (TA376385) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using FUS antibody (TA376385) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunohistochemistry of paraffin-embedded human breast cancer using FUS antibody (TA376385) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat brain using FUS antibody (TA376385) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse brain using FUS antibody (TA376385) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse spleen using FUS antibody (TA376385) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Immunofluorescence analysis of C6 cells using FUS antibody (TA376385) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of NIH/3T3 cells using FUS antibody (TA376385) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug FUS antibody (TA376385). Western blot was performed from the immunoprecipitate using FUS antibody (TA376385) at a dilition of 1:2000.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53kDa
• Gene Name: FUS RNA binding protein
• Database Link:
  Entrez Gene 2521 Human
  UniProt ID P35637
• Background: This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.
• Synonyms: ALS6; FUS1; hnRNP-P2; POMp75; TLS