GNAI3 Rabbit Polyclonal Antibody

CAT#: TA376636

GNAI3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody (TA376636) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using GNAI3 antibody (TA376636) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of L929 cells using [KO Validated] GNAI3 Rabbit pAb (TA376636) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GNAI3 antibody (TA376636). Western blot was performed from the immunoprecipitate using GNAI3 antibody (TA376636) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:100
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40kDa
• Gene Name: G protein subunit alpha i3
• Database Link:
  Entrez Gene 2773 Human
  UniProt ID P08754
• Background: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.
• Synonyms: 87U6; FLJ26559