H2BC21 Rabbit Polyclonal Antibody

CAT#: TA377053

MonoMethyl-Histone H2B-K5 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of C6 cells, using MonoMethyl-Histone H2B-K5 antibody (TA377053) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H2B-K5 antibody (TA377053) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H2B-K5 antibody (TA377053) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H2B-K5 antibody (TA377053) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H2B-K5 antibody (TA377053) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: MonoMethyl K5
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of Human H2BK5me1.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 13kDa
• Gene Name: histone cluster 2, H2be
• Database Link:
  Entrez Gene 8349 Human
  UniProt ID Q16778
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Aug 2015]
• Synonyms: GL105; H2B; H2B-GL105; H2B.1; H2B.q; H2B/q; H2BFQ; H2BGL105; H2BQ; MGC119802; MGC119804; MGC129733; MGC129734