H3FT (HIST3H3) Rabbit Polyclonal Antibody

CAT#: TA377063

Acetyl-Histone H3-K27 Rabbit polyclonal Antibody

Specifications

Product Data

• Applications: ChIP, IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
  ChIP,1:20 - 1:100
  ChIP-seq,1:20 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Acetyl K27
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 15kDa
• Gene Name: histone cluster 3 H3
• Database Link:
  Entrez Gene 8290 Human
  UniProt ID P68431
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.