HSF1 Rabbit Polyclonal Antibody

CAT#: TA377242

Phospho-HSF1-S326 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of MCF7 cells, using Phospho-HSF1-S326 antibody (TA377242) at 1:500 dilution.MCF7 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human
• Modifications: Phospho S326
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding S326 of human HSF1
• Formulation: Buffer: PBS with 0.02% sodium azide,1% BSA,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,1% BSA,50% glycerol,pH7.3.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 82kDa
• Gene Name: heat shock transcription factor 1
• Database Link:
  Entrez Gene 3297 Human
  UniProt ID Q00613
• Background: The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016]
• Synonyms: HSTF1