SHIP (INPP5D) Rabbit Polyclonal Antibody

CAT#: TA377517

Phospho-SHIP1-Y1020 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Raji cells, using Phospho-SHIP1-Y1020 antibody (TA377517) at 1:1000 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human
• Modifications: Phospho Y1020
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y1020 of human SHIP1 (NP_001017915.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 109kDa/133kDa
• Gene Name: inositol polyphosphate-5-phosphatase D
• Database Link:
  Entrez Gene 3635 Human
  UniProt ID Q92835
• Background: This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants.
• Synonyms: hp51CN; MGC104855; MGC142140; MGC142142; p150Ship; SHIP; SHIP-1; SHIP1; SIP-145