CD11b (ITGAM) Rabbit Polyclonal Antibody

CAT#: TA377608

CD11b/ITGAM Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CD11b/ITGAM antibody (TA377608) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of TF-1 cells using CD11b/ITGAM Rabbit pAb (TA377608) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of THP-1 cells using CD11b/ITGAM Rabbit pAb (TA377608) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD11b/ITGAM (NP_001139280.1).
• Formulation: Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 127kDa
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
  UniProt ID P11215
• Background: This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6