JAK1 Rabbit Polyclonal Antibody

CAT#: TA377644

JAK1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using JAK1 antibody (TA377644) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Western blot analysis of extracts of Mouse lung, using JAK1 antibody (TA377644) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] JAK1 Rabbit pAb (TA377644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using [KO Validated] JAK1 Rabbit pAb (TA377644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using [KO Validated] JAK1 Rabbit pAb (TA377644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human JAK1.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 133kDa
• Gene Name: Janus kinase 1
• Database Link:
  Entrez Gene 3716 Human
  UniProt ID P23458
• Background: This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants.
• Synonyms: JAK-1; JAK1A; JAK1B; JTK3