SAM68 (KHDRBS1) Rabbit Polyclonal Antibody

CAT#: TA377775

KHDRBS1/Sam68 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using KHDRBS1/Sam68 antibody (TA377775) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunohistochemistry of paraffin-embedded rat brain using KHDRBS1/Sam68 antibody (TA377775) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human prostate cancer using KHDRBS1/Sam68 antibody (TA377775) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human stomach using KHDRBS1/Sam68 antibody (TA377775) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse liver using KHDRBS1/Sam68 antibody (TA377775) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Confocal immunofluorescence analysis of HeLa cells using KHDRBS1/Sam68 Polyclonal Antibody (TA377775) at dilution of 1:400. Blue: DAPI for nuclear staining.

• Confocal immunofluorescence analysis of U2OS cells using KHDRBS1/Sam68 Polyclonal Antibody (TA377775) at dilution of 1:400. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:100 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human KHDRBS1/Sam68 (NP_006550.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa/45kDa/48kDa
• Gene Name: KH RNA binding domain containing, signal transduction associated 1
• Database Link:
  Entrez Gene 10657 Human
  UniProt ID Q07666
• Background: This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.
• Synonyms: FLJ34027; p62; p68; SAM68