myosin heavy chain 9 (MYH9) Rabbit Polyclonal Antibody

CAT#: TA378838

MYH9 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MYH9 antibody (TA378838) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 15s.

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  Immunohistochemistry of paraffin-embedded human esophagus using MYH9 antibody (TA378838) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human stomach using MYH9 antibody (TA378838) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MYH9 antibody (TA378838). Western blot was performed from the immunoprecipitate using MYH9 antibody (TA378838) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  IHC,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9 (NP_002464.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 159kDa/226kDa
• Gene Name: myosin, heavy chain 9, non-muscle
• Database Link:
  Entrez Gene 4627 Human
  UniProt ID P35579
• Background: This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
• Synonyms: DFNA17; EPSTS; FTNS; MGC104539; MHA; NMHC-II-A; NMMHC-A; NMMHC-IIA; NMMHCA