NBR1 Rabbit Polyclonal Antibody

CAT#: TA378943

NBR1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Mouse testis, using NBR1 Rabbit pAb (TA378943) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

• 

  Immunohistochemistry of paraffin-embedded human liver, using NBR1 antibody (TA378943).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunofluorescence analysis of NIH/3T3 cells using NBR1 Rabbit pAb (TA378943) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of PC-12 cells using NBR1 Rabbit pAb (TA378943) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunoprecipitation analysis of extracts of K562 cells, using NBR1 antibody (TA378943).

Show all 5 images

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:100
  IP,1:20 - 1:50
• Reactivities: Human, Mouse, Rat, Monkey
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human NBR1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 103kDa/107kDa
• Gene Name: NBR1, autophagy cargo receptor
• Database Link:
  Entrez Gene 4077 Human
  UniProt ID Q14596
• Background: The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants.
• Synonyms: 1A1-3B; 1A13B; CA125; FLJ55359; FLJ98272; KIAA0049; M17S2; MIG19