PABP (PABPC1) Rabbit Polyclonal Antibody

CAT#: TA379564

polyclonal AntibodyPC1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PABPC1 antibody (TA379564) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

• 

  Immunohistochemistry of paraffin-embedded rat testis using PABPC1 antibody (TA379564) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunohistochemistry of paraffin-embedded human esophageal using PABPC1 antibody (TA379564) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunohistochemistry of paraffin-embedded mouse testis using PABPC1 antibody (TA379564) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunofluorescence analysis of L929 cells using PABPC1 Polyclonal Antibody (TA379564) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of U-2 OS cells using PABPC1 Polyclonal Antibody (TA379564) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Show all 6 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 61kDa/70kDa
• Gene Name: poly(A) binding protein cytoplasmic 1
• Database Link:
  Entrez Gene 26986 Human
  UniProt ID P11940
• Background: This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
• Synonyms: PAB1; PABP; PABP1; PABPC2; PABPL1