PDHA1 Rabbit Polyclonal Antibody

CAT#: TA379753

Phospho-PDHA1-S293 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-PDHA1-S293 antibody (TA379753) at 1:1000 dilution.293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S293
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding S293 of human PDHA1
• Formulation: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40kDa
• Gene Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Database Link:
  Entrez Gene 5160 Human
  UniProt ID P08559
• Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
• Synonyms: PDHA; PDHCE1A; PHE1A