Protein Phosphatase 1 beta (PPP1CB) Rabbit Polyclonal Antibody

CAT#: TA380231

PP1 beta Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PP1 beta antibody (TA380231) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 60s.

• 

  Immunohistochemistry of paraffin-embedded rat brain using PP1 beta Rabbit pAb (TA380231) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry of paraffin-embedded human placenta using PP1 beta Rabbit pAb (TA380231) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry of paraffin-embedded mouse stomach using PP1 beta Rabbit pAb (TA380231) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• 

  Immunofluorescence analysis of U2OS cells using PP1 beta Rabbit pAb (TA380231) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of L929 cells using PP1 beta Rabbit pAb (TA380231) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of H9C2 cells using PP1 beta Rabbit pAb (TA380231) at dilution of 1:100. Blue: DAPI for nuclear staining.

Show all 7 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human PP1 beta (NP_002700.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 37kDa
• Gene Name: protein phosphatase 1 catalytic subunit beta
• Database Link:
  Entrez Gene 5500 Human
  UniProt ID P62140
• Background: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Synonyms: MGC3672; OTTHUMP00000200968; PP-1B; PPP1CD