GLP1 (GCG) Rabbit Polyclonal Antibody

CAT#: TA380395

Pro-glucagon Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Pro-glucagon antibody (TA380395) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunohistochemistry of paraffin-embedded mouse pancreas using Pro-glucagon Rabbit pAb (TA380395) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat pancreas using Pro-glucagon Rabbit pAb (TA380395) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of rat pancreas using Pro-glucagon antibody (TA380395) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of mouse pancreas using Pro-glucagon antibody (TA380395) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-180 of human Pro-glucagon (NP_002045.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 20kDa
• Gene Name: glucagon
• Database Link:
  Entrez Gene 2641 Human
  UniProt ID P01275
• Background: The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
• Synonyms: GLP1; GLP2; glucagon; GRPP