PSMD13 Rabbit Polyclonal Antibody

CAT#: TA380465

PSMD13 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PSMD13 antibody (TA380465) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

• 

  Immunohistochemistry of paraffin-embedded human liver damage using PSMD13 antibody (TA380465) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunohistochemistry of paraffin-embedded mouse lung using PSMD13 antibody (TA380465) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunofluorescence analysis of C6 cells using PSMD13 Polyclonal Antibody (TA380465) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of HeLa cells using PSMD13 Polyclonal Antibody (TA380465) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Show all 5 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa
• Gene Name: proteasome 26S subunit, non-ATPase 13
• Database Link:
  Entrez Gene 5719 Human
  UniProt ID Q9UNM6
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described.
• Synonyms: HSPC027; p40.5; Rpn9; S11