RSK1 p90 (RPS6KA1) Rabbit Polyclonal Antibody

CAT#: TA381120

p90Rsk/RSK1/RPS6KA1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using p90Rsk/RSK1/RPS6KA1 antibody (TA381120) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunohistochemistry of paraffin-embedded mouse spleen using RPS6KA1 antibody (TA381120) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of L929 cells using RPS6KA1 antibody (TA381120) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using RPS6KA1 antibody (TA381120) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:100 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 72kDa/81kDa/82kDa/83kDa
• Gene Name: ribosomal protein S6 kinase A1
• Database Link:
  Entrez Gene 6195 Human
  UniProt ID Q15418
• Background: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Synonyms: HU-1; MAPKAPK1A; p90S6K; pp90RSK1; RSK; RSK-1; RSK1