RSPO1 Rabbit Polyclonal Antibody

CAT#: TA381173

RSPO1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Rat pancreas, using RSPO1 antibody (TA381173) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

• 

  Western blot analysis of extracts of SKOV3 cells, using RSPO1 antibody (TA381173) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

• 

  Immunohistochemistry of paraffin-embedded rat ovary using RSPO1 Rabbit pAb (TA381173) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• 

  Immunofluorescence analysis of A549 cells using RSPO1 Rabbit pAb (TA381173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of C6 cells using RSPO1 Rabbit pAb (TA381173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of NIH-3T3 cells using RSPO1 Rabbit pAb (TA381173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Show all 6 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-263 of human RSPO1 (NP_001033722.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21kDa/25kDa/28kDa
• Gene Name: R-spondin 1
• Database Link:
  Entrez Gene 284654 Human
  UniProt ID Q2MKA7
• Background: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
• Synonyms: CRISTIN3; FLJ40906; hRspo1; OTTHUMP00000180612; OTTHUMP00000180613; R-spondin1; RSPO; RSPONDIN