SMC3 Rabbit Polyclonal Antibody

CAT#: TA381772

SMC3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SMC3 antibody (TA381772) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

• 

  Immunoprecipitation analysis of 600ug extracts of Mouse spleen using 3ug SMC3 antibody (TA381772). Western blot was performed from the immunoprecipitate using SMC3 antibody (TA381772) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:50 - 1:200
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SMC3.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 141kDa
• Gene Name: structural maintenance of chromosomes 3
• Database Link:
  Entrez Gene 9126 Human
  UniProt ID Q9UQE7
• Background: This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
• Synonyms: BAM; bamacan; BMH; CDLS3; CSPG6; HCAP; SMC3L1