Smoothened (SMO) Rabbit Polyclonal Antibody

CAT#: TA381790

Phospho-SMO-S611/615/616 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using Phospho-SMO-S611/615/616 antibody (TA381790) at 1:1000 dilution.293T-SMO cells were treated by SHH (0.4ug/ml) at 37℃ after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 1min.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:1000
• Reactivities: Human
• Modifications: Phospho S611/615/616
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S611/615/616 of human SMO.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 86kDa
• Gene Name: smoothened, frizzled class receptor
• Database Link:
  Entrez Gene 6608 Human
  UniProt ID Q99835
• Background: The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
• Synonyms: Gx; SMOH; smoothened