SOAT 1 (SOAT1) Rabbit Polyclonal Antibody

CAT#: TA381868

SOAT1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SOAT1 antibody (TA381868) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of U2OS cells using SOAT1 Rabbit pAb (TA381868) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using SOAT1 Rabbit pAb (TA381868) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of C6 cells using SOAT1 Rabbit pAb (TA381868) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57kDa/58kDa/64kDa
• Gene Name: sterol O-acyltransferase 1
• Database Link:
  Entrez Gene 6646 Human
  UniProt ID P35610
• Background: The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene.
• Synonyms: ACACT; ACACT1; ACAT; ACAT-1; ACAT1; RP11-215I23.2; SOAT; STAT