KAP1 (TRIM28) Rabbit Polyclonal Antibody

CAT#: TA382816

KAP1/TRIM28 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using KAP1/KAP1/TRIM28 antibody (TA382816) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunohistochemistry of paraffin-embedded human breast cancer using KAP1/KAP1/TRIM28 Rabbit pAb (TA382816) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human esophageal cancer using KAP1/KAP1/TRIM28 Rabbit pAb (TA382816) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse spinal cord using KAP1/KAP1/TRIM28 Rabbit pAb (TA382816) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat ovary using KAP1/KAP1/TRIM28 Rabbit pAb (TA382816) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of C6 cells using KAP1/KAP1/TRIM28 Polyclonal Antibody (TA382816) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of L929 cells using KAP1/KAP1/TRIM28 Polyclonal Antibody (TA382816) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of U-2 OS cells using KAP1/KAP1/TRIM28 Polyclonal Antibody (TA382816) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KAP1/TRIM28 antibody (TA382816). Western blot was performed from the immunoprecipitate using KAP1/TRIM28 antibody (TA382816) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:1000 - 1:3000
  IHC,1:100 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human KAP1/KAP1/TRIM28 (NP_005753.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 79kDa/88kDa
• Gene Name: tripartite motif containing 28
• Database Link:
  Entrez Gene 10155 Human
  UniProt ID Q13263
• Background: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
• Synonyms: FLJ29029; KAP-1; KAP1; KRIP-1; RNF96; TF1B; TIF1-beta; TIF1B