Tuberin (TSC2) Rabbit Polyclonal Antibody

CAT#: TA382887

Phospho-TSC2-S1387 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of C2C12 cells, using Phospho-TSC2-S1387 Rabbit pAb (TA382887) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S1387
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding S1387 of human Tuberin/TSC2.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 25kDa/187-200kDa
• Gene Name: tuberous sclerosis 2
• Database Link:
  Entrez Gene 7249 Human
  UniProt ID P49815
• Background: Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: FLJ43106; LAM; TSC4; tuberin