Sts1 (UBASH3B) Rabbit Polyclonal Antibody

CAT#: TA382990

UBASH3B Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using UBASH3B antibody (TA382990) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

• 

  Immunohistochemistry of paraffin-embedded rat spleen using UBASH3B antibody (TA382990) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunofluorescence analysis of A549 cells using UBASH3B antibody (TA382990). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human UBASH3B (NP_116262.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 72kDa
• Gene Name: ubiquitin associated and SH3 domain containing B
• Database Link:
  Entrez Gene 84959 Human
  UniProt ID Q8TF42
• Background: This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor.
• Synonyms: KIAA1959; MGC15437; p70; STS-1; STS1