Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: TA383206

Vinculin Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Vinculin antibody (TA383206) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of PC-12 cells using Vinculin antibody (TA383206) at dilution of 1:150. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using Vinculin antibody (TA383206) at dilution of 1:150. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using Vinculin antibody (TA383206) at dilution of 1:150. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 767-1066 of human Vinculin (NP_003364.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa/116kDa/123kDa
• Gene Name: vinculin
• Database Link:
  Entrez Gene 7414 Human
  UniProt ID P18206
• Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
• Synonyms: CMD1W; metavinculin; MVCL; vinculin