VCIP 135 (VCPIP1) Rabbit Polyclonal Antibody

CAT#: TA383208

VCPIP1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using VCPIP1 antibody (TA383208) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 923-1222 of human VCPIP1 (NP_079330.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 134kDa
• Gene Name: valosin containing protein interacting protein 1
• Database Link:
  Entrez Gene 80124 Human
  UniProt ID Q96JH7
• Background: Deubiquitinating enzyme involved in DNA repair and reassembly of the Golgi apparatus and the endoplasmic reticulum following mitosis. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity. Plays a role in VCP-mediated formation of transitional endoplasmic reticulum (tER (By similarity. Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity. Also involved in DNA repair following phosphorylation by ATM or ATR: acts by catalyzing deubiquitination of SPRTN, thereby promoting SPRTN recruitment to chromatin and subsequent proteolytic cleavage of covalent DNA-protein cross-links (DPCs. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.
• Synonyms: DKFZp686G038; DUBA3; FLJ23132; FLJ60694; KIAA1850; VCIP135