VDAC2 Rabbit Polyclonal Antibody

CAT#: TA383211

VDAC2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using VDAC2 antibody (TA383211) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunohistochemistry of paraffin-embedded human brain using VDAC2 Rabbit pAb (TA383211) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human breast cancer using VDAC2 Rabbit pAb (TA383211) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse testis using VDAC2 Rabbit pAb (TA383211) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat heart using VDAC2 Rabbit pAb (TA383211) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of C6 cells using VDAC2 Rabbit pAb (TA383211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of NIH-3T3 cells using VDAC2 Rabbit pAb (TA383211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VDAC2 (NP_003366.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 30kDa/31kDa/33kDa
• Gene Name: voltage dependent anion channel 2
• Database Link:
  Entrez Gene 7417 Human
  UniProt ID P45880
• Background: This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants.
• Synonyms: FLJ23841; hVDAC2; VDAC-2