VIRMA Rabbit Polyclonal Antibody

CAT#: TA383247S

KIAA1429 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using KIAA1429 antibody (TA383247) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunohistochemistry of paraffin-embedded rat brain using KIAA1429 antibody (TA383247) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human thyroid cancer using KIAA1429 antibody (TA383247) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human placenta using KIAA1429 antibody (TA383247) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse brain using KIAA1429 antibody (TA383247) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1700 of human KIAA1429 (NP_056311.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 128kDa/154kDa/201kDa/202kDa
• Gene Name: KIAA1429
• Database Link:
  Entrez Gene 25962 Human
  UniProt ID Q69YN4
• Background: Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs in the 3'-UTR near the stop codon: recruits the catalytic core components METTL3 and METTL14, thereby guiding m6A methylation at specific sites. Required for mRNA polyadenylation via its role in selective m6A methylation: m6A methylation of mRNAs in the 3'-UTR near the stop codon correlating with alternative polyadenylation (APA.
• Synonyms: DKFZP434I116; DKFZp781B2117; fSAP121; MGC138493; MGC141940; MSTP054