VPS36 Rabbit Polyclonal Antibody

CAT#: TA383261S

VPS36 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using VPS36 antibody (TA383261) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of NIH-3T3 cells using VPS36 Polyclonal Antibody (TA383261) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-220 of human VPS36 (NP_057159.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 36kDa/43kDa
• Gene Name: vacuolar protein sorting 36 homolog
• Database Link:
  Entrez Gene 51028 Human
  UniProt ID Q86VN1
• Background: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II.
• Synonyms: C13orf9; CGI-145; DKFZp781E0871; EAP45