WIPI2 Rabbit Polyclonal Antibody

CAT#: TA383323

WIPI2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using WIPI2 antibody (TA383323) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 3s.

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  Western blot analysis of extracts of Rat testis, using WIPI2 antibody (TA383323) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_057087.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa/46kDa/47kDa/48kDa/49kDa
• Gene Name: WD repeat domain, phosphoinositide interacting 2
• Database Link:
  Entrez Gene 26100 Human
  UniProt ID Q9Y4P8
• Background: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]
• Synonyms: Atg21; CGI-50; DKFZP434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WIPI-2