XPC Rabbit Polyclonal Antibody

CAT#: TA383375

XPC Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using XPC antibody (TA383375) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 5s.

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  Immunohistochemistry of paraffin-embedded human colon carcinoma using XPC Rabbit pAb (TA383375) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug XPC antibody ( TA383375). Western blot was performed from the immunoprecipitate using XPC antibody ( TA383375) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IP,1:50 - 1:100
• Reactivities: Human
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 700-940 of human XPC (NP_004619.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 15kDa/101kDa/105kDa
• Gene Name: XPC complex subunit, DNA damage recognition and repair factor
• Database Link:
  Entrez Gene 7508 Human
  UniProt ID Q01831
• Background: This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.
• Synonyms: p125; RAD4; XP3; XPCC