Ku70 (XRCC6) Rabbit Polyclonal Antibody

CAT#: TA383388

Ku70 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Ku70 antibody (TA383388) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunohistochemistry of paraffin-embedded rat brain using Ku70 antibody (TA383388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human gastric cancer using Ku70 antibody (TA383388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human uterus using Ku70 antibody (TA383388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of A549 cells using Ku70 antibody (TA383388). Blue: DAPI for nuclear staining.

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  Confocal immunofluorescence analysis of U2OS cells using Ku70 Polyclonal Antibody (TA383388) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug Ku70 antibody (TA383388). Western blot was performed from the immunoprecipitate using Ku70 antibody (TA383388) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
  IP,1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human Ku70 (NP_001460.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 65kDa/69kDa
• Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 6
• Database Link:
  Entrez Gene 2547 Human
  UniProt ID P12956
• Background: The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
• Synonyms: CTC75; CTCBF; D22S671; D22S731; G22P1; KU70; ML8; OTTHUMP00000199333; OTTHUMP00000199349; TLAA