14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

CAT#: TA383424

YWHAZ Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using YWHAZ Antibody (TA383424) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunohistochemistry of paraffin-embedded Rat kidney using YWHAZ Rabbit pAb (TA383424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of Rat brain using YWHAZ Rabbit pAb (TA383424) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human YWHAZ (NP_001129171.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 19kDa/27kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  Entrez Gene 7534 Human
  UniProt ID P63104
• Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
• Synonyms: 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; OTTHUMP00000165851; OTTHUMP00000165858