YY1 Rabbit Polyclonal Antibody

CAT#: TA383425

YY1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using YY1 antibody (TA383425) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (TA383425) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Specifications

Product Data

• Applications: ChIP, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  ChIP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YY1 (NP_003394.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa
• Gene Name: YY1 transcription factor
• Database Link:
  Entrez Gene 7528 Human
  UniProt ID P25490
• Background: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
• Synonyms: DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1; YY-1