alpha Actinin (ACTN1) Rabbit Polyclonal Antibody

CAT#: TA383672

Sarcomeric alpha Actinin Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:1(4°,overnight). High-pressure and temperature Tris-EDTA,pH8. was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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  Western Blot analysis of various cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1：1000

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  Western Blot analysis of A549 cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1：1000

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  Western blot analysis of lysates from 293, HeLa, and A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/20000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACTN1/2/3/4. AA range:21-70
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):75
• Gene Name: actinin alpha 1
• Database Link:
  Entrez Gene 87 Human
  UniProt ID P12814
• Background: Swiss-Prot Acc.P12814/P35609/Q08043/O43707.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.
• Synonyms: FLJ40884; FLJ54432