AKT3 Rabbit Monoclonal Antibody [Clone ID: R08-8F1]

CAT#: TA383697

AKT Rabbit monoclonal Antibody

Product Images

Western blot detection of AKT1/2/3 in LnCap cell lysates using AKT Rabbit mAb(1:500 diluted).Predicted band size:56kDa.Observed band size:56kDa.

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  Immunocytochemistry of AKT1/2/3(green) in Hela cells using AKT Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R08-8F1
• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human AKT1/2/3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 56 kDa; Observed MW: 56 kDa
• Gene Name: AKT serine/threonine kinase 3
• Database Link:
  Entrez Gene 10000 Human
  UniProt ID Q9Y243
• Background: Swiss-Prot Acc.Q9Y243.AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
• Synonyms: DKFZp434N0250; OTTHUMP00000037911; OTTHUMP00000037912; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2