Alkaline Phosphatase (ALPL) Rabbit Polyclonal Antibody

CAT#: TA383714

Alkaline Phosphatase Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.

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  Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .

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  Western blot analysis of Alkaline Phosphatase expression in (1)HepG2 cell lysate; (2)JAR cell lysate.

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  Western blot analysis of extracts from SKOV-3, Jurkat and MCF7 cells using Alkaline Phosphatase Rabbit pAb at 1:1000 dilution. Predicted band size: 57kDa. Observed band size: 70kDa.

Specifications

Product Data

• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50-1/200
  ICC/IF: 1/50-1/100
  IP: 1/50
  FC: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human Alkaline Phosphatase
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 57kDa
• Gene Name: alkaline phosphatase, liver/bone/kidney
• Database Link:
  Entrez Gene 249 Human
  UniProt ID P05186
• Background: Swiss-Prot Acc.P05186.There are at least four distinct but related alkaline phosphatases: inTinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.
• Synonyms: AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; MGC161443; MGC167935; OTTHUMP00000002972; TNAP; TNSALP