ATG5 Mouse Monoclonal Antibody [Clone ID: 3C10-5C8-8C3]

CAT#: TA383769

APG5L (3C10) Mouse monoclonal Antibody

Product Images

Western blot detection of Atg5/Apg5L (3C1) in C2C12,K562,C6,Mouse brain cell lysates using Atg5/Apg5L (3C1) antibody. Observed band size:55kDa.

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  Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 Mouse mAb diluted at 1:200.

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  Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 Mouse mAb diluted at 1:200.

Specifications

Product Data

• Clone Name: 3C10-5C8-8C3
• Applications: IHC, WB
• Recommended Dilution: WB: 1/5000-10000
  IHC: 1/100-200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant protein expressed in E.coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 55kDa
• Gene Name: autophagy related 5
• Database Link:
  Entrez Gene 9474 Human
  UniProt ID Q9H1Y0
• Background: Swiss-Prot Acc.Q9H1Y0.Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
• Synonyms: APG5; APG5-LIKE; APG5L; ASP; hAPG5; OTTHUMP00000017824