ATG5 Rabbit Monoclonal Antibody [Clone ID: R05-4F2]

CAT#: TA383771

APG5L Rabbit monoclonal Antibody

Product Images

Western blot detection of APG5L/ATG5 Rabbit pA in K562,C6,Hela cell lysates using APG5L/ATG5 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:55kDa.

Specifications

Product Data

• Clone Name: R05-4F2
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  ICC/IF: 1/20-1/100
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human APG5L/ATG5
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 33 kDa; Observed MW: 55 kDa
• Gene Name: autophagy related 5
• Database Link:
  Entrez Gene 9474 Human
  UniProt ID Q9H1Y0
• Background: Swiss-Prot Acc.Q9H1Y0.Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
• Synonyms: APG5; APG5-LIKE; APG5L; ASP; hAPG5; OTTHUMP00000017824