ATG7 Mouse Monoclonal Antibody [Clone ID: 1F8-8H4-4G10]

CAT#: TA383772

ATG7 (1F8) Mouse monoclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG7 Mouse mAb diluted at 1:200.

Specifications

Product Data

• Clone Name: 1F8-8H4-4G10
• Applications: IHC
• Recommended Dilution: IHC: 1/100-200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant protein expressed in E.coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 78kDa
• Gene Name: autophagy related 7
• Database Link:
  Entrez Gene 10533 Human
  UniProt ID O95352
• Background: Swiss-Prot Acc.O95352.E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
• Synonyms: APG7-LIKE; APG7L; DKFZp434N0735; GSA7; hAGP7