ATG7 Rabbit Monoclonal Antibody [Clone ID: R01-6E1]

CAT#: TA383775

ATG7 Rabbit monoclonal Antibody

Product Images

Western blot detection of ATG7 in K562 cell lysates using ATG7 Rabbit mAb(1:1000 diluted).Predicted band size:78kDa.Observed band size:78kDa.

Specifications

Product Data

• Clone Name: R01-6E1
• Applications: IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human ATG7
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 78 kDa; Observed MW: 78 kDa
• Gene Name: autophagy related 7
• Database Link:
  Entrez Gene 10533 Human
  UniProt ID O95352
• Background: Swiss-Prot Acc.O95352.E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
• Synonyms: APG7-LIKE; APG7L; DKFZp434N0735; GSA7; hAGP7